Auto Immune & Metabolic Disease Models
Multiple Sclerosis Mouse Model: EAE
MS is a CNS inflammatory disease caused by an autoimmune response against the myelin sheath insulation of nerve fibers, results in loss of motor-neuron control.
In mice, MOG peptide induces an auto-immune response, resulting in demyelination of nerve cells. Hindlimb and forelimb paralysis ensues and is scored/quantified.
SC injection of 0.2mg MOG35-55 (myelin oligodendrocyte glycoprotein 35-55) in 0.4mg killed mycobacterium tuberculosis H37Ra, 0.1ml at 2 sites on the back at time= 0 hours.
IP injection of 600ng pertussis toxin at time= 2 hours and again at time= 22-26 hours
Hindlimb/forelimb scoring of paralysis, scale 0-5
CD20, IL-17, GM-CSF analysis
Collagen Induced Arthritis Model
CIA model driven by adaptive immune responses, includes presence of rheumatoid factor or anticitrullinated peptide antibody
Adjuvant induced models rely on innate and T-cell responses
Recombinant human Glucose-6-phosphate isomerase (rh-GPI) induces T-cell dependent inflammatory arthritis in genetically susceptible mice (male DBA/1)
Th2-driven model, sustained T-cell help to B cells leads to the production of high-affinity IgG1 antibodies against G6PI, arthritogenic
Animals are injected SC at the base of the tail (2 sites of injection, 100µl/site), using a 27g needle with 400µg of rhGPI or collagen that has been emulsified 1:1 with CFA
Scoring of footpad swelling: from 0 = normal, 1 = erythema and slight swelling of the major joint, 2 = moderate to severe swelling of the major joint, 3 = severe swelling of the entire paw.
IL-17, IL-1β, TNF, CD20 analysis
Systemic Lupus Erythematosus
NZBWF1 Transgenic model
MRL/lpr Transgenic model
Pristane Inducible Lupus model
Metabolic Disease Models
ob/ob leptin deficient mouse and db/db mouse model for diabetic dyslipidemia
Oral Glucose Tolerance Test